Speaker
Gergely Papp
(EMBL)
Description
Preparing biological samples for cryo-imaging applications requires fine control of cooling rate and sample thickness. I will present our latest instrumentation developments aimed at vitrifying purified protein solutions and cells for subsequent cryo-imaging experiments. Our automated machine comprises a plasma generation module to render the sample support hydrophilic, picoliter drop dispenser heads to deliver the biological sample onto the sample support, and a liquid ethane jet for rapid freezing of the specimen. After vitrification, the samples are stored in cryogenic conditions using cold nitrogen gas.
